Potassium Transporter KUP7 Is Involved in K(+) Acquisition and Translocation in Arabidopsis Root under K(+)-Limited Conditions.

Autor: Han M; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China., Wu W; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China., Wu WH; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China., Wang Y; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China. Electronic address: yiwang@cau.edu.cn.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2016 Mar 07; Vol. 9 (3), pp. 437-446. Date of Electronic Publication: 2016 Feb 04.
DOI: 10.1016/j.molp.2016.01.012
Abstrakt: Potassium (K(+)) is one of the essential macronutrients for plant growth and development. K(+) uptake from environment and K(+) translocation in plants are conducted by K(+) channels and transporters. In this study, we demonstrated that KT/HAK/KUP transporter KUP7 plays crucial roles in K(+) uptake and translocation in Arabidopsis root. The kup7 mutant exhibited a sensitive phenotype on low-K(+) medium, whose leaves showed chlorosis symptoms compared with wild-type plants. Loss of function of KUP7 led to a reduction of K(+) uptake rate and K(+) content in xylem sap under K(+)-deficient conditions. Thus, the K(+) content in kup7 shoot was significantly reduced under low-K(+) conditions. Localization analysis revealed that KUP7 was predominantly targeted to the plasma membrane. The complementation assay in yeast suggested that KUP7 could mediate K(+) transport. In addition, phosphorylation on S80, S719, and S721 was important for KUP7 activity. KUP7 was ubiquitously expressed in many organs/tissues, and showed a higher expression level in Arabidopsis root. Together, our data demonstrated that KUP7 is crucial for K(+) uptake in Arabidopsis root and might be also involved in K(+) transport into xylem sap, affecting K(+) translocation from root toward shoot, especially under K(+)-limited conditions.
(Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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