GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex.

Autor:	Anastasiades PG; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.; Centre for Neuroscience, Imperial College Faculty of Medicine, Hammersmith Hospital, Imperial College London, London W12 0NN, UK., Marques-Smith A; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK., Lyngholm D; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK., Lickiss T; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK., Raffiq S; Centre for Neuroscience, Imperial College Faculty of Medicine, Hammersmith Hospital, Imperial College London, London W12 0NN, UK., Kätzel D; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.; Department of Clinical and Experimental Epilepsy, Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK., Miesenböck G; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.; Centre for Neural Circuits and Behaviour, University of Oxford, Oxford OX1 3SR, UK., Butt SJ; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2016 Feb 04; Vol. 7, pp. 10584. Date of Electronic Publication: 2016 Feb 04.
DOI:	10.1038/ncomms10584
Abstrakt:	GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find that Nkx2-1-derived interneurons possess functional synaptic connections before emerging pyramidal cell networks. Subsequent interneuron circuit maturation is both subtype and layer dependent. Glutamatergic input onto fast spiking (FS), but not somatostatin-positive, non-FS interneurons increases over development. Interneurons of both subtype located in layers (L) 4 and 5b engage in transient circuits that disappear after the somatosensory critical period. These include a pathway mediated by L5b somatostatin-positive interneurons that specifically targets L4 during the first postnatal week. The innervation patterns of immature cortical interneuron circuits are thus neither static nor progressively strengthened but follow a layer-specific choreography of transient connections that differ from those of the adult brain.
Databáze:	MEDLINE
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