Effect of oleic acid on store-operated calcium entry in immune-competent cells.

Autor: Carrillo C; Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain. ccarrillo@ubu.es., Giraldo M; Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain., Cavia MM; Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain., Alonso-Torre SR; Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain.
Jazyk: angličtina
Zdroj: European journal of nutrition [Eur J Nutr] 2017 Apr; Vol. 56 (3), pp. 1077-1084. Date of Electronic Publication: 2016 Jan 30.
DOI: 10.1007/s00394-016-1157-5
Abstrakt: Purpose: To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca 2+ entry (SOCE) is a Ca 2+ influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca 2+ signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE.
Methods: Changes in [Ca 2+ ] i were measured using the Fura-2 fluorescence dye. Mn 2+ uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca 2+ -insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells.
Results: We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca 2+ ] i partly mediated by an extracellular Ca 2+ influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the concentration tested, thus intensifying the physiological relevance of our findings.
Conclusion: We suggest a physiological pathway for the beneficial effects of OA in inflammation.
Databáze: MEDLINE
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