Use of Aloe vera-based extender for chilling and freezing collared peccary (Pecari tajacu) semen.
Autor: | Souza AL; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Lima GL; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Peixoto GC; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Silva AM; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Oliveira MF; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Silva AR; Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil. Electronic address: legio2000@yahoo.com. |
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Jazyk: | angličtina |
Zdroj: | Theriogenology [Theriogenology] 2016 May; Vol. 85 (8), pp. 1432-8. Date of Electronic Publication: 2016 Jan 12. |
DOI: | 10.1016/j.theriogenology.2016.01.007 |
Abstrakt: | As an alternative for the conservation of collared peccary semen, this research aims at evaluating the use of Aloe vera (AV) extract as a cryoprotectant for semen chilling and freezing. Five ejaculates were divided in two aliquots that were diluted in Tris plus egg yolk (EY; 20%) or AV extract (20%) and chilled at 5 °C. In both treatments, an adequate semen conservation was achieved and values closer to 40% motile sperm with viability and osmotic response ranging from 20% to 40%, and normal morphology of 80% were found after 36 hours of storage. Moreover, 12 other ejaculates were diluted in Tris plus EY (20%) or AV extract (5, 10, or 20%) and glycerol (3%). Samples were frozen in liquid nitrogen and thawed after 1 week. After thawing, all the treatments containing EY or AV provided similar values for sperm morphology, viability, osmotic response, membrane integrity, sperm motility, amplitude of lateral head, beat cross frequency, and rapid, low, and static subpopulations, but the highest values for straightness and the lowest values for curvilinear velocity were found using 20% AV (P < 0.05). In conclusion, we found that AV extract at a 20% concentration could be used as an alternative substitute to EY in the formulation of Tris extenders for collared peccaries' semen chilling or freezing. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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