Relationship between regulatory T cells subsets and lipid profile in dyslipidemic patients: a longitudinal study during atorvastatin treatment.

Autor: Guasti L; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. luigina.guasti@uninsubria.it., Maresca AM; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. andrea.maresca@uninsubria.it.; Department of Clinical and Experimental Medicine, University of Insubria, Viale Borri 57, Varese, 21100, Italy. andrea.maresca@uninsubria.it., Schembri L; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. laura.schembri@uninsubria.it., Rasini E; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. emanuela.rasini@uninsubria.it., Dentali F; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. francesco.dentali@uninsubria.it., Squizzato A; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. alessandro.squizzato@uninsubria.it., Klersy C; Biometry and Clinical Epidemiology, Research Department, Foundation IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy. catherine.klersy@libero.it., Robustelli Test L; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. lalu86@tiscali.it., Mongiardi C; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. mongiardi@gmail.com., Campiotti L; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. leonard.campiotto@uninsubria.it., Ageno W; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. walter.agen@uninsubria.it., Grandi AM; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. anna.grandi@uninsubria.it., Cosentino M; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. marco.cosentino@uninsubria.it., Marino F; Research Center on Dyslipidemia, University of Insubria, Varese, Italy. franca.marino@uninsubria.it.
Jazyk: angličtina
Zdroj: BMC cardiovascular disorders [BMC Cardiovasc Disord] 2016 Jan 29; Vol. 16, pp. 26. Date of Electronic Publication: 2016 Jan 29.
DOI: 10.1186/s12872-016-0201-y
Abstrakt: Background: The CD4+ T-lymphocytes and their subtype CD4 + CD25(high)FoxP3+ regulatory T cells are receiving growing interest as major regulators of atherogenesis. We sought to investigate 1) whether the CD4 + cell subsets were expressed differently in dyslipidemic patients (Pts) and healthy subjects (HS) and 2) whether atorvastatin treatment could be associated in-vivo and in-vitro with cell changes in expression and functional response.
Methods: CD4+ subsets frequency (CD4 + CD25(high)FoxP3+, CD4 + CD25-FoxP3+) and mRNA expression for FoxP3, IL-10 and TGF-β were evaluated in 30 consecutive Pts at baseline and after a 3-month atorvastatin therapy, and in 17 HS.
Results: The % of CD4 + cells did not differ between HS and Pts. The % of CD4 + CD25(high)FoxP3+ was higher in Pts than HS and did not change during treatment. The CD4 + CD25-FoxP3+ cells were similar between the two groups and were lower in Pts at visit 2. Cytokine expression and FoxP3 did not differ in HS and Pts and no substantial change was observed during treatment. At visit 1, CD4 + CD25(high)FoxP3+ cells were significantly correlated with both total-cholesterol (r = 0.570, P = 0.0002), LDL-cholesterol (r = 0.715, P = 0.0001), Apolipoprotein B (r = 0.590, P = 0.0001). In-vitro atorvastatin (up to 5 μM) failed to induce any significant modulation of cell functions.
Conclusion: CD4 + CD25(high)FoxP3+ regulatory cells seem to be over-stimulated in the early pre-clinical phase of atherosclerosis and a relationship exists between their frequency and circulating lipids. A potential immuno-modulation by statin treatment is not achieved through a normalization in peripheral CD4 + cell subsets.
Databáze: MEDLINE
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