| Autor: |
Ul'masov TN, Gulov MK, Aliev KA, Andrianov VM, Piruzian ES |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 1989 Aug (8), pp. 17-20. |
| Abstrakt: |
The fragments of cotton Gossipium hirsutum c.v. 108-f chloroplast genome were cloned in Escherichia coli cells. The cloned psbA and rbcL genes have been selected using the heterologous probes from spinach. The preliminary attempts to clone the complete psbA gene in pUC19 vector failed, probably, due to the toxicity of its product to Escherichia coli cells, and its 5'- and 3'-ends were cloned separately. Reconstruction experiments revealed that while the complete psbA gene was unable to be stably inherited by Escherichia coli cells, its structural part lacking the promoter region could be readily cloned in the bacterial cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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