Dexmedetomidine and ketamine show distinct patterns of cell degeneration and apoptosis in the developing rat neonatal brain.
| Autor: | Pancaro C; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and., Segal BS; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and., Sikes RW; b Division of Movement and Rehabilitation Sciences , Department of Physical Therapy, Northeastern University , Boston , MA USA., Almeer Z; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and., Schumann R; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and., Azocar RJ; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and., Marchand JE; a Department of Anesthesiology , Tufts School of Medicine, Tufts Medical Center , Boston , MA , USA and. |
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| Jazyk: | angličtina |
| Zdroj: | The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians [J Matern Fetal Neonatal Med] 2016 Dec; Vol. 29 (23), pp. 3827-33. Date of Electronic Publication: 2016 Mar 08. |
| DOI: | 10.3109/14767058.2016.1148132 |
| Abstrakt: | Objective: Early exposure to common anesthetic and sedative agents causes widespread brain cell degeneration and apoptosis in the developing rat brain, associated with persistent learning deficits in rats. This study was designed to determine whether the α2 adrenergic receptor agonist, dexmedetomidine, produces brain cell degeneration and apoptosis in postnatal day-7 rats in the same brain areas when compared to ketamine. Methods: Systemic saline, ketamine 20 mg/kg, or dexmedetomidine at 30 or 45 μg/kg were given six times to postnatal day 7 rats (n = 6/group) every 90 min. Twenty-four hours after the initial injection, brain regions were processed and analyzed for cell degeneration using the silver stain and for apoptosis using activated caspase-3 immunohistochemistry. Results: Exposure to ketamine resulted in significant cellular degeneration and apoptosis in limbic brain regions, but nonsignificant changes in primary sensory brain regions. In contrast, dexmedetomidine produced significant cellular degeneration and apoptosis in primary sensory brain regions, but nonsignificant changes in limbic regions. Conclusions: These data show that ketamine and dexmedetomidine result in anatomically distinct patterns of cell degeneration and apoptosis in the brains of 7-day-old rat pups. The meaning and the clinical significance of these findings remain to be established. |
| Databáze: | MEDLINE |
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