[Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci].

Autor:	Dong Y, Lin SS, Cao Y, Wu WW, Huang SQ, Zheng WG, Li FY, Ge BW, Guo YL, Zhou HG
Jazyk:	čínština
Zdroj:	Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2015 Oct; Vol. 31 (5), pp. 373-6, 380.
Abstrakt:	Objective: To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice. Methods: Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed. Results: A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion: The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
Databáze:	MEDLINE
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