Prevalence of bla(PenA) and bla(OXA) in Burkholderia pseudomallei Isolated from Patients at Sappasitthiprasong Hospital and Their Susceptibility to Ceftazidime and Carbapenems.
| Autor: | Panya M, Thirat S, Wanram S, Panomket P, Nilsakul J |
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| Jazyk: | angličtina |
| Zdroj: | Journal of the Medical Association of Thailand = Chotmaihet thangphaet [J Med Assoc Thai] 2016 Jan; Vol. 99 Suppl 1, pp. S12-6. |
| Abstrakt: | Background: Burkholderia pseudomallei is a causative agent of melioidosis. Ceftazidime is the preferred drug of choice for treatment. However, the motility rate is high in endemic areas. Objective: This study aimed to determine the susceptibility tofour different antimicrobial agents and to detect the β-lactamase genes in B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital. Material and Method: 85 B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital between November 2010 and May 2011 were determined for antimicrobial susceptibility by standard disk diffusion and minimum inhibitory concentration (MIC). Real-time polymerase chain reaction (PCR) was used for the detection of bla(penA) and bla(OXA) in β-lactamase genes. Results: Almost all of the clinical isolates ofB. pseudomallei were susceptible to ceftazidime and imipenem. Cefatzidime MIC was ≤ 1-16 μg/ml and imipenem MIC was ≤ 1-4 μg/ml. The real-time PCR revealed that more than 90% of B. pseudomallei isolates carried bla(PenA) and bla(OXA). Conclusion: Although the clinical isolates of B. pseudomallei were susceptible to ceftazidime and imipenem, this study showed B. pseudomallei had a gene that produced beta-lactamase enzyme and may be poorly effective in the use of beta-lactam drugs. |
| Databáze: | MEDLINE |
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