| Autor: |
Pennington HG; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom., Gheorghe DM; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom., Damerum A; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom., Pliego C; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom., Spanu PD; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom., Cramer R; Department of Chemistry, University of Reading , Reading RG6 6AD, United Kingdom., Bindschedler LV; Department of Chemistry, University of Reading , Reading RG6 6AD, United Kingdom.; School of Biological Sciences, Royal Holloway University of London , Egham TW20 0EX, United Kingdom. |
| Abstrakt: |
There are over 500 candidate secreted effector proteins (CSEPs) or Blumeria effector candidates (BECs) specific to the barley powdery mildew pathogen Blumeria graminis f.sp. hordei. The CSEP/BEC proteins are expressed and predicted to be secreted by biotrophic feeding structures called haustoria. Eight BECs are required for the formation of functional haustoria. These include the RNase-like effector BEC1054 (synonym CSEP0064). In order to identify host proteins targeted by BEC1054, recombinant BEC1054 was expressed in E. coli, solubilized, and used in pull-down assays from barley protein extracts. Many putative interactors were identified by LC-MS/MS after subtraction of unspecific binders in negative controls. Therefore, a directed yeast-2-hybrid assay, developed to measure the effectiveness of the interactions in yeast, was used to validate putative interactors. We conclude that BEC1054 may target several host proteins, including a glutathione-S-transferase, a malate dehydrogenase, and a pathogen-related-5 protein isoform, indicating a possible role for BEC1054 in compromising well-known key players of defense and response to pathogens. In addition, BEC1054 interacts with an elongation factor 1 gamma. This study already suggests that BEC1054 plays a central role in barley powdery mildew virulence by acting at several levels. |
