Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status.

Autor:	Hamilton TR; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., de Castro LS; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Delgado Jde C; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., de Assis PM; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Siqueira AF; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Mendes CM; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Goissis MD; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Muiño-Blanco T; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Cebrián-Pérez JÁ; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Nichi M; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., Visintin JA; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain., D'Ávila Assumpção ME; Department of Animal ReproductionSchool of Veterinary Medicine and Animal Science, University of Sao Paulo. Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, BrazilGrupo de Biología y Fisiología de la ReproducciónFacultad de Veterinaria, Instituto de Investigación de Ciencias Ambientales de Aragón (IUCA), Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain meoaa@usp.br.
Jazyk:	angličtina
Zdroj:	Reproduction (Cambridge, England) [Reproduction] 2016 Apr; Vol. 151 (4), pp. 379-90. Date of Electronic Publication: 2016 Jan 25.
DOI:	10.1530/REP-15-0403
Abstrakt:	Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm. (© 2016 Society for Reproduction and Fertility.)
Databáze:	MEDLINE
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