Biofortified indica rice attains iron and zinc nutrition dietary targets in the field.

Autor: Trijatmiko KR; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor 16111, Indonesia., Dueñas C; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Tsakirpaloglou N; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Torrizo L; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Arines FM; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Adeva C; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Balindong J; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Oliva N; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Sapasap MV; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Borrero J; Centro Internacional de Agricultura Tropical, Cali, Colombia., Rey J; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Francisco P; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Nelson A; Social Sciences Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.; Faculty of Geo-Information and Earth Observation (ITC), University of Twente, Enschede 7500 AE, The Netherlands., Nakanishi H; Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan., Lombi E; Centre for Environmental Risk Assessment and Remediation, University of South Australia, Australia., Tako E; United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Cornell University, New York., Glahn RP; United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Cornell University, New York., Stangoulis J; School of Biological Sciences, Flinders University of South Australia, Adelaide, Australia., Chadha-Mohanty P; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Johnson AA; School of Botany, The University of Melbourne, Victoria 3010, Australia., Tohme J; Centro Internacional de Agricultura Tropical, Cali, Colombia., Barry G; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines., Slamet-Loedin IH; Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.; Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911, Indonesia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2016 Jan 25; Vol. 6, pp. 19792. Date of Electronic Publication: 2016 Jan 25.
DOI: 10.1038/srep19792
Abstrakt: More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g(-1) iron (Fe) and 16 μg g(-1) zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g(-1) Fe and 28 μg g(-1) Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g(-1) in glasshouse settings; in contrast, under field conditions, 4 μg g(-1) was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g(-1) Fe and 45.7 μg g(-1) Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.
Databáze: MEDLINE