Evaluation of photodynamic therapy on fibroblast viability and cytokine production.

Autor: Gomes-Filho JE; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: joao@foa.unesp.br., Sivieri-Araujo G; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: gustavosivieri@uol.com.br., Sipert CR; Department of Restorative Dentistry, Discipline of Endodontics, Dental School, University of São Paulo, São Paulo, SP, Brazil. Electronic address: carlasipert@gmail.com., da Silva Santos LM; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: ludmillasantos@yahoo.com.br., de Azevedo Queiroz ÍO; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: indiaodonto@gmail.com., Men Martins C; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: christinemen@hotmail.com., do Carmo Maia NK; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: kivilla@hotmail.com., Cintra LTA; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: lucianocintra@foa.unesp.br., Dezan-Junior E; Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: dezan@foa.unesp.br., Bagnato VS; Optics Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, Brazil. Electronic address: vander@ifsc.usp.br., Chaves-Neto AH; Department of Basic Science, Discipline of Biochemistry, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: antoniohernandes@foa.unesp.br., de Oliveira SHP; Department of Basic Science, Discipline of Pharmacology, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: shpoliv@foa.unesp.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2016 Mar; Vol. 13, pp. 97-100. Date of Electronic Publication: 2016 Jan 18.
DOI: 10.1016/j.pdpdt.2016.01.007
Abstrakt: Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1β and IL-6) production by mouse fibroblasts.
Methods: Sixty seconds of pre-irradiation time with curcumin 500mg/L and Led wavelength (λ) 480nm, 72Jcm(2), for 300s was used for PDT. Solutions were diluted in culture medium DMEM (1×10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6, 24 and 48h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p<0.05) for MTT and Kruskal-Wallis test and Dunn (p<0.05) for ELISA.
Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p>0.05) while 5% NaOCl was more cytotoxic than PDT (p<0.05) in all periods of time. All materials similarly expressed IL-1β and IL-6 regardless to the experimental period (p<0.05).
Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1β and IL-6.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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