| Autor: |
Jimenez HJ; Programa de Pós-Graduação em Agronomia/Melhoramento Genético de Plantas Universidade Federal Rural de Pernambuco, Recife, PE, Brasil., Martins LS; Programa de Pós-Graduação em Agronomia/Melhoramento Genético de Plantas Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.; Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil., Montarroyos AV; Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil., Silva Junior JF; Empresa Brasileira de Pesquisa Agropecuária, Aracaju, SE, Brasil., Alzate-Marin AL; Programa de Pós-Graduação em Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brasil., Moraes Filho RM; Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil. |
| Abstrakt: |
Mangabeira (Hancornia speciosa Gomes) is a fruit tree of the Apocynaceae family, which is native to Brazil and is a very important food resource for human populations in its areas of occurrence. Mangabeira fruit is collected as an extractive activity, and no domesticated varieties or breeding programs exist. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The objective of this study was to characterize and evaluate the genetic diversity of 38 mangabeira individuals collected from natural populations in Pernambuco State using inter-simple sequence repeat (ISSR) molecular markers. The ISSR methodology generated a total of 93 loci; 10 were monomorphic and 83 were polymorphic. The average number of loci per primer was 15.5, ranging from 9 (#UBC 866) to 21 (#UBC 834). The results showed a high level of genetic diversity (0.30), and found that only around 30% of genetic variability is distributed among populations (GST = 0.29, ФST = 0.30), with the remainder (ФCT = 70%) found within each population, as expected for forest outcrossing species. Estimates for historic gene flow (1.18) indicate that there is some isolation of these populations, and some degree of genetic differentiation. |
