Barcoding markers for Pneumocystis species in wildlife.

Autor: Danesi P; Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy. Electronic address: pdanesi@izsvenezie.it., da Rold G; Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy., Rizzoli A; Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige 38010, Italy., Hauffe HC; Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige 38010, Italy., Marangon S; Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy., Samerpitak K; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; CBS-KNAW Fungal Biodiversity Centre, Utrecht 3584CT, The Netherlands., Demanche C; Department of Parasitology, Faculty of Biological and Pharmaceutical Sciences, University of Lille, Lille 59006, France; Center for Infection and Immunity of Lille, INSERM, Institut Pasteur de Lille, Lille 59006, France., Guillot J; Department of Parasitology-Mycology, Dynamyc Research Group, École Nationale Vétérinaire d'Alfort, UPEC, Maisons-Alfort 94700, France., Capelli G; Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy., de Hoog SG; CBS-KNAW Fungal Biodiversity Centre, Utrecht 3584CT, The Netherlands; Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam 1090GE, The Netherlands.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2016 Feb; Vol. 120 (2), pp. 191-206. Date of Electronic Publication: 2015 Sep 05.
DOI: 10.1016/j.funbio.2015.08.019
Abstrakt: Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis.
(Copyright © 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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