| Autor: |
Pires CT, de L Scodro RB, Brenzan MA, Cortez DA, Siqueira VL, Cardozo-Filho L, Goncalves RM, Caleffi-Ferracioli KR, Cardoso RF; Department of Clinical Analysis and Biomedicine, State University of Maringa. Av. Colombo, 5790, CEP 87020-900 Maringa, Parana, Brazil. rfressatticardoso@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2016; Vol. 17 (6), pp. 532-9. |
| DOI: |
10.2174/1389201017666160114094723 |
| Abstrakt: |
The conventional techniques used to extract natural products have many disadvantages, and alternative methods have been used, such as supercritical fluid extraction (SFE-CO2). We compared the anti-Mycobacterium tuberculosis activity and cytotoxicity of extracts and major pure compounds were obtained from the leaves of Calophyllum brasiliense by SFE-CO2, maceration and Soxhlet. Anti-M tuberculosis activity was evaluated by resazurin microtiter assay plate and cytotoxicity assay was performed using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide. The (-) mammea A/BB, (-) mammea B/BB, mammea B/BB cyclo D, ponnalide, mammea A/BA cyclo D, and amentoflavone were identified as the majority compounds. SFE-CO2, especially at 313 K and 10.92 MPa showed better yield for (-) mammea A/BB. Anti-M. tuberculosis activity (62.5 μg/mL) and cytotoxicity (Selectivity Index = 0.320-0.576) were similar for the three extracts. Mammea B/BB cyclo D had a minimum inhibitory concentration (MIC) of 125 μg/mL, and ponnalide and mammea A/BA cyclo D had MICs > 250 μg/mL. The pure compounds isolated showed low Selectivity Index (< 0.09). SFE-CO2 may be more promising than conventional methods for the extraction of compound (-) mammea A/BB, which presented the best anti-M. tuberculosis activity in our previous study. This is important for current industrial requirements to obtain extracts from medicinal plants using clean technologies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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