Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation.

Autor:	McPherson AD; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA., Barrios JP; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA., Luks-Morgan SJ; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA., Manfredi JP; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA., Bonkowsky JL; Department of Pediatrics, University of Utah, Salt Lake City, UT 84112, USA., Douglass AD; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA. Electronic address: adam.douglass@neuro.utah.edu., Dorsky RI; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA. Electronic address: richard.dorsky@neuro.utah.edu.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2016 Jan 25; Vol. 26 (2), pp. 263-269. Date of Electronic Publication: 2016 Jan 07.
DOI:	10.1016/j.cub.2015.11.064
Abstrakt:	Postembryonic neurogenesis has been observed in several regions of the vertebrate brain, including the dentate gyrus and rostral migratory stream in mammals, and is required for normal behavior [1-3]. Recently, the hypothalamus has also been shown to undergo continuous neurogenesis as a way to mediate energy balance [4-10]. As the hypothalamus regulates multiple functional outputs, it is likely that additional behaviors may be affected by postembryonic neurogenesis in this brain structure. Here, we have identified a progenitor population in the zebrafish hypothalamus that continuously generates neurons that express tyrosine hydroxylase 2 (th2). We develop and use novel transgenic tools to characterize the lineage of th2(+) cells and demonstrate that they are dopaminergic. Through genetic ablation and optogenetic activation, we then show that th2(+) neurons modulate the initiation of swimming behavior in zebrafish larvae. Finally, we find that the generation of new th2(+) neurons following ablation correlates with restoration of normal behavior. This work thus identifies for the first time a population of dopaminergic neurons that regulates motor behavior capable of functional recovery. (Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze:	MEDLINE
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