Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen.

Autor: Kaysheva AL; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia. Electronic address: kaysheva@gmail.com., Ivanov YD; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Frantsuzov PA; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Krohin NV; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Pavlova TI; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Uchaikin VF; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Konev VА; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Kovalev OB; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Ziborov VS; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia., Archakov AI; Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2016 Mar; Vol. 229, pp. 86-90. Date of Electronic Publication: 2016 Jan 07.
DOI: 10.1016/j.jviromet.2015.12.012
Abstrakt: A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force microscope (AFM chip) with subsequent protein identification by tandem mass spectrometric (MS/MS) analysis. Biospecific AFM-capturing of viral particles containing HCVcoreAg from serum samples was performed by use of AFM chips with monoclonal antibodies (anti-HCVcore) covalently immobilized on the surface. Biospecific complexes were registered and counted by AFM. Further MS/MS analysis allowed to reliably identify the HCVcoreAg in the complexes formed on the AFM chip surface. Analysis of MS/MS spectra, with the account taken of the possible polymorphisms in the amino acid sequence of the HCVcoreAg, enabled us to increase the number of identified peptides.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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