NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity.

Autor: Gupta DK; Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Prof. Albareda No 1, Granada, 18008, Spain., Pena LB; Department of Biological Chemistry, Faculty of Pharmacy and Biochemistry, IQUIFIB, CONICET, University of Buenos Aires, Buenos Aires, C1113AAD, Argentina., Romero-Puertas MC; Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Prof. Albareda No 1, Granada, 18008, Spain., Hernández A; Postgrados de Agronomía, Universidad Centroccidental Lisandro Alvarado, Apdo 400, Barquisimeto, 3001, Venezuela., Inouhe M; Department of Biology, Faculty of Science, Ehime University, Matsuyama, 790-8577, Japan., Sandalio LM; Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Prof. Albareda No 1, Granada, 18008, Spain.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2017 Apr; Vol. 40 (4), pp. 509-526. Date of Electronic Publication: 2016 Apr 01.
DOI: 10.1111/pce.12711
Abstrakt: The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohF, which were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. Cadmium reduced the growth of leaves in WT, AtrbohC and D, but not in AtrbohF. A time-dependent increase in H 2 O 2 and lipid peroxidation was observed in all genotypes, with AtrbohC showing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated in AtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally in AtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1 day of treatment and even after 5 days in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.
(© 2016 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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