Association of myelin peptide with vitamin D prevents autoimmune encephalomyelitis development.

Autor: Mimura LA; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., Chiuso-Minicucci F; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., Fraga-Silva TF; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., Zorzella-Pezavento SF; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., França TG; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., Ishikawa LL; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil., Penitenti M; Flow Cytometry Laboratory - Dr. Amaral Carvalho Foundation, Rua Dona Silvéria, 150, Jaú, São Paulo 17210-070, Brazil., Ikoma MR; Flow Cytometry Laboratory - Dr. Amaral Carvalho Foundation, Rua Dona Silvéria, 150, Jaú, São Paulo 17210-070, Brazil., Sartori A; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Univ. Estadual Paulista (UNESP), Distrito de Rubião Junior, Botucatu, São Paulo 18618-970, Brazil. Electronic address: sartori@ibb.unesp.br.
Jazyk: angličtina
Zdroj: Neuroscience [Neuroscience] 2016 Mar 11; Vol. 317, pp. 130-40. Date of Electronic Publication: 2016 Jan 04.
DOI: 10.1016/j.neuroscience.2015.12.053
Abstrakt: Multiple sclerosis is a chronic, inflammatory and demyelinating disease of the central nervous system (CNS). As there is no cure for this disease, new therapeutic strategies and prophylactic measures are necessary. We recently described the therapeutic activity of the association between myelin oligodendrocyte glycoprotein peptide (MOG) and active vitamin D3 (VitD) against experimental autoimmune encephalomyelitis (EAE). The objective of this work was to evaluate the prophylactic potential of this association in EAE. C57BL/6 mice were vaccinated with MOG in the presence of VitD and then subjected to EAE induction. Animals were euthanized 7 and 19days after disease induction and the following parameters were evaluated: body weight, clinical score, inflammatory process in the CNS, amount of dendritic cells (DCs) and regulatory T cells in the spleen and cytokine production by spleen and CNS cell cultures. Vaccination with MOG associated with VitD determined a drastic reduction in clinical score, body weight loss, CNS inflammation, DCs maturation and also in the production of cytokines by CNS and spleen cell cultures. Collectively, our data indicate that this association prevents EAE development. A similar effect from specific self-antigens associated with VitD is expected in other autoimmune conditions and deserves to be experimentally appraised.
(Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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