A stereospecific solid-phase screening assay for colonies expressing both (R)- and (S)-selective ω-aminotransferases.
| Autor: | Willies SC; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK., Galman JL; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK., Slabu I; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK., Turner NJ; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK nicholas.turner@manchester.ac.uk. |
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| Jazyk: | angličtina |
| Zdroj: | Philosophical transactions. Series A, Mathematical, physical, and engineering sciences [Philos Trans A Math Phys Eng Sci] 2016 Feb 28; Vol. 374 (2061). |
| DOI: | 10.1098/rsta.2015.0084 |
| Abstrakt: | A novel solid-phase screening assay was developed for colonies expressing both (R)- and (S)-selective ω-aminotransferases. This high-throughput assay can be used to screen rapidly large variant libraries with enhanced substrate selectivity and enantioselectivities. (© 2016 The Author(s).) |
| Databáze: | MEDLINE |
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