Real-time monitoring of the metabolic capacity of ex vivo rat olfactory mucosa by proton transfer reaction mass spectrometry (PTR-MS).

Autor: Schoumacker R; CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.; INRA, UMR1324 CSGA, F-21000, Dijon, France.; Université de Bourgogne, UMR CSGA, F-21000, Dijon, France., Robert-Hazotte A; CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.; INRA, UMR1324 CSGA, F-21000, Dijon, France.; Université de Bourgogne, UMR CSGA, F-21000, Dijon, France., Heydel JM; CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.; INRA, UMR1324 CSGA, F-21000, Dijon, France.; Université de Bourgogne, UMR CSGA, F-21000, Dijon, France., Faure P; CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.; INRA, UMR1324 CSGA, F-21000, Dijon, France.; Université de Bourgogne, UMR CSGA, F-21000, Dijon, France., Le Quéré JL; CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France. jllequere@dijon.inra.fr.; INRA, UMR1324 CSGA, F-21000, Dijon, France. jllequere@dijon.inra.fr.; Université de Bourgogne, UMR CSGA, F-21000, Dijon, France. jllequere@dijon.inra.fr.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2016 Feb; Vol. 408 (6), pp. 1539-43. Date of Electronic Publication: 2016 Jan 11.
DOI: 10.1007/s00216-015-9289-7
Abstrakt: Olfactory mucosa (OM) can metabolise odorant volatile organic compounds through various enzymatic mechanisms to produce odorous or non-odorous metabolites. Preliminary ex vivo studies using headspace-gas chromatography (HS-GC) revealed the formation of metabolites when odorant molecules were injected in the headspace above a fresh explant of rat olfactory mucosa. However, this method did not allow accessing the data during the first 5 min of contact between the odorant and the mucosa; thus limiting the olfactory biological significance. Using a direct-injection mass spectrometry technique with a proton transfer reaction instrument (PTR-MS), we have been able, for the first time, to investigate the first moments of the enzymatic process of the metabolic capacity of ex vivo rat olfactory mucosa in real time. Using ethyl acetate as a model volatile odorous substrate, we demonstrated here for the first time that this odorant could be metabolised by an ex vivo olfactory mucosa within seconds, producing ethanol as metabolite.
Databáze: MEDLINE
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