Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.

Autor: Angermueller C; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK., Clark SJ; Epigenetics Programme, Babraham Institute, Cambridge, UK., Lee HJ; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Macaulay IC; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Teng MJ; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Hu TX; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.; Medical Research Council Functional Genomics Unit, University of Oxford, UK., Krueger F; Bioinformatics Group, Babraham Institute, Cambridge, UK., Smallwood S; Epigenetics Programme, Babraham Institute, Cambridge, UK., Ponting CP; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.; Medical Research Council Functional Genomics Unit, University of Oxford, UK., Voet T; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.; Department of Human Genetics, Katholieke Universiteit (KU) Leuven, Leuven, Belgium., Kelsey G; Epigenetics Programme, Babraham Institute, Cambridge, UK., Stegle O; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK., Reik W; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2016 Mar; Vol. 13 (3), pp. 229-232. Date of Electronic Publication: 2016 Jan 11.
DOI: 10.1038/nmeth.3728
Abstrakt: We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
Databáze: MEDLINE
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