Effect of steroid hormones on sulfated oviductal glycoprotein secretion by oviductal explants in vitro.

Autor: Erickson-Lawrence MF; Department of Anatomy, University of Virginia Charlottesville 22908., Turner TT, Thomas TS, Oliphant G
Jazyk: angličtina
Zdroj: Biology of reproduction [Biol Reprod] 1989 Jun; Vol. 40 (6), pp. 1311-9.
DOI: 10.1095/biolreprod40.6.1311
Abstrakt: Explants of rabbit ampullary and isthmic tissue were cultured 4 days with and without exogenous steroids, and the sulfated oviductal glycoprotein (SOG) concentration in the explant culture supernatants was determined. Tissues cultured with progesterone plus estrogen secreted significantly more SOG than control tissues, whereas tissues cultured with estrogen alone did not. Ampullary tissues cultured with progesterone plus estrogen secreted significantly more SOG than control tissues on Days 2 and 3, whereas SOG secretion by isthmic tissues was significantly above control secretion on Day 4. Ampullary and isthmic tissues differed significantly in their secretory capacity. Maximum ampullary SOG secretion was approximately 650 ng SOG/mg tissue/day. Maximum isthmic SOG secretion was approximately 30 ng SOG/mg tissue/day. These findings suggest that the oviduct is composed of discrete functional regions that provide support to gametes and developing embryos through the unique secretory characteristics of each region.
Databáze: MEDLINE