| Autor: |
Quecine MC; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Leite TF; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Bini AP; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Regiani T; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Franceschini LM; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Budzinski IG; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Marques FG; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Labate MT; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Guidetti-Gonzalez S; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Moon DH; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil., Labate CA; Departament of Genetics, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba -SP, Brazil. |
| Abstrakt: |
Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. |
