| Autor: |
Nadeau RW, Oldfield NF, Garland WA, Liberato DJ |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 1989 Aug 01; Vol. 61 (15), pp. 1732-6. |
| DOI: |
10.1021/ac00190a028 |
| Abstrakt: |
A sensitive and specific assay for recombinant interleukin-2 (rIL-2) in human serum is described. The assay is based on a sequential sandwich immunobioassay that uses a microtiter plate coated with anti-rIL-2 monoclonal antibody (specific for recombinant human IL-2) to capture rIL-2 from serum, and an IL-2 dependent T-cell line that proliferates in a dose-dependent fashion. The lower limit of quantitation of the assay is 2 units/mL (1 unit = approximately 50 pg) using 0.1 mL of serum and the calibration curves ranged from 2 to 50 units/mL. Data are reported on the sensitivity, precision, reproducibility, and specificity of the assay; the stability of rIL-2 in serum; and the recovery of rIL-2 from serum. We also report on the use of the procedure to assay clinical samples from patients with AIDS undergoing treatment with rIL-2. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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