In vivo wound healing activity of gels containing Cecropia pachystachya leaves.

Autor: Duque AP; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Pinto Nde C; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Mendes Rde F; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., da Silva JM; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Aragão DM; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Castañon MC; Department of Morphology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Scio E; Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Jazyk: angličtina
Zdroj: The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2016 Jan; Vol. 68 (1), pp. 128-38. Date of Electronic Publication: 2016 Jan 04.
DOI: 10.1111/jphp.12496
Abstrakt: Objectives: To evaluate the in-vivo wound healing and in-vitro antioxidant activity of gels containing the ethyl acetate extract of Cecropia pachystachya (ECP) 2% and 5%, and to perform the chemical fingerprint of ECP by HPLC-DAD.
Methods: The wound healing activity of the gels was evaluated for 21 days, using the excision model in rats followed by histopathological and histomorphometric analysis. The in-vitro antioxidant effect of ECP was investigated using 1-diphenyl-2-picrylhydrazyl, reducing power, β-carotene bleaching and thiobarbituric acid reactive substances assays. Also, a HPLC analysis was performed to identify the chemical markers orientin, iso-orientin and chlorogenic acid.
Key Findings: The group of animals treated with ECP 5% presented oedema and inflammatory infiltrate with less intensity than the other groups. Both ECP 2% and 5% gels showed less neovascularization and cellularity, and better tissue repair when compared to the control, which showed a younger and homogeneous tissue.
Conclusions: This study had demonstrated that the ECP gels promoted the acceleration of the healing process when compared to the control group. Wound contraction, angiogenesis, epithelialization and the collagen deposition support further evaluation of C. pachystachya leaves in the topical treatment and management of skin wounds.
(© 2015 Royal Pharmaceutical Society.)
Databáze: MEDLINE
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