Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid.

Autor: Abdallah MB; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Karray F; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia. fatma.karray@cbs.rnrt.tn.; Laboratoire Mixte International « Contaminants et Ecosystèmes Marins Sud Méditerranéens » (LMI COSYS-Med), Sfax, Tunisia. fatma.karray@cbs.rnrt.tn., Mhiri N; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.; Laboratoire Mixte International « Contaminants et Ecosystèmes Marins Sud Méditerranéens » (LMI COSYS-Med), Sfax, Tunisia., Mei N; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Quéméneur M; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Cayol JL; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Erauso G; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.; Laboratoire Mixte International « Contaminants et Ecosystèmes Marins Sud Méditerranéens » (LMI COSYS-Med), Sfax, Tunisia., Tholozan JL; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Alazard D; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.; IRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France., Sayadi S; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.; Laboratoire Mixte International « Contaminants et Ecosystèmes Marins Sud Méditerranéens » (LMI COSYS-Med), Sfax, Tunisia.
Jazyk: angličtina
Zdroj: Extremophiles : life under extreme conditions [Extremophiles] 2016 Mar; Vol. 20 (2), pp. 125-38. Date of Electronic Publication: 2016 Jan 02.
DOI: 10.1007/s00792-015-0805-7
Abstrakt: Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 × 10(6) DNA copies g(-1) whereas archaea varied between 5 × 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid.
Databáze: MEDLINE
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