Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis.
| Autor: | Kabytaev K; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Durairaj A; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Shin D; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Rohlfing CL; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Connolly S; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Little RR; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA., Stoyanov AV; University of Missouri, School of Medicine, One Hospital Drive, Columbia, MO, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of separation science [J Sep Sci] 2016 Feb; Vol. 39 (4), pp. 676-81. Date of Electronic Publication: 2016 Jan 28. |
| DOI: | 10.1002/jssc.201500989 |
| Abstrakt: | A liquid chromatography with mass spectrometry on-line platform that includes the orthogonal techniques of ion exchange and reversed phase chromatography is applied for C-peptide analysis. Additional improvement is achieved by the subsequent application of cation- and anion-exchange purification steps that allow for isolating components that have their isoelectric points in a narrow pH range before final reversed-phase mass spectrometry analysis. The utility of this approach for isolating fractions in the desired "pI window" for profiling complex mixtures is discussed. (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| Databáze: | MEDLINE |
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