Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells.
| Autor: | Badertscher L; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland; Molecular Life Sciences Ph.D. Program, 8057 Zurich, Switzerland., Wild T; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland., Montellese C; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland; Molecular Life Sciences Ph.D. Program, 8057 Zurich, Switzerland., Alexander LT; Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland., Bammert L; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland; Molecular Life Sciences Ph.D. Program, 8057 Zurich, Switzerland., Sarazova M; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland; Molecular Life Sciences Ph.D. Program, 8057 Zurich, Switzerland., Stebler M; RNAi Screening Center, ScopeM, ETH Zurich, 8093 Zurich, Switzerland., Csucs G; RNAi Screening Center, ScopeM, ETH Zurich, 8093 Zurich, Switzerland., Mayer TU; Department of Biology, University of Konstanz, 78457 Konstanz, Germany., Zamboni N; Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland., Zemp I; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland., Horvath P; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland; RNAi Screening Center, ScopeM, ETH Zurich, 8093 Zurich, Switzerland., Kutay U; Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland. Electronic address: ulrike.kutay@bc.biol.ethz.ch. |
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| Jazyk: | angličtina |
| Zdroj: | Cell reports [Cell Rep] 2015 Dec 29; Vol. 13 (12), pp. 2879-91. Date of Electronic Publication: 2015 Dec 17. |
| DOI: | 10.1016/j.celrep.2015.11.061 |
| Abstrakt: | Ribosome biogenesis is a highly complex process requiring many assisting factors. Studies in yeast have yielded comprehensive knowledge of the cellular machinery involved in this process. However, many aspects of ribosome synthesis are different in higher eukaryotes, and the global set of mammalian ribosome biogenesis factors remains unexplored. We used an imaging-based, genome-wide RNAi screen to find human proteins involved in 40S ribosomal subunit biogenesis. Our analysis identified ∼ 300 factors, many part of essential protein modules such as the small subunit (SSU) processome, the eIF3 and chaperonin complexes, and the ubiquitin-proteasome system. We demonstrate a role for the vertebrate-specific factor RBIS in ribosome synthesis, uncover a requirement for the CRL4 E3 ubiquitin ligase in nucleolar ribosome biogenesis, and reveal that intracellular glutamine synthesis supports 40S subunit production. (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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