The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation.

Autor:	Landi A; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium., Timermans CG; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium., Naessens E; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium., Vanderstraeten H; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium., Stove V; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium., Verhasselt B; Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium.
Jazyk:	angličtina
Zdroj:	The Journal of general virology [J Gen Virol] 2016 Mar; Vol. 97 (3), pp. 778-785. Date of Electronic Publication: 2015 Dec 22.
DOI:	10.1099/jgv.0.000382
Abstrakt:	Human immunodeficiency virus type 1 (HIV-1)-mediated CD4 downregulation is an important determinant of viral replication in vivo. Research on cellular co-factors involved in this process could lead to the identification of potential therapeutic targets. We found that CD4 surface levels were significantly higher in HIV-1-infected cells knocked-down for the HIV Rev-binding protein (HRB) compared with control cells. HRB knock-down affected CD4 downregulation induced by Nef but not by HIV-1 Vpu. Interestingly, the knock-down of the related protein HRBL (HRB-like), but not of the HRB interaction partner EPS15 (epidermal growth factor receptor pathway substrate 15), increased CD4 levels in Vpu-expressing cells significantly. Both of these proteins are known to be involved in HIV-1-mediated CD4 downregulation as co-factors of HIV-1 Nef. These results identify HRB as a previously unknown co-factor for HIV-1 Nef-mediated CD4 downregulation and highlight differences with the related protein HRBL, which affects the CD4 downregulation in a dual role as co-factor of both HIV-1 Nef and Vpu.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu