Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides.
| Autor: | Beine B; Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany., Diehl HC; Clinical Proteomics, Medizinisches Proteom-Center, Ruhr-University, Bochum, Germany., Meyer HE; Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany., Henkel C; Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany. corinna.henkel@isas.de. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1394, pp. 129-150. |
| DOI: | 10.1007/978-1-4939-3341-9_10 |
| Abstrakt: | Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a technique to visualize molecular features of tissues based on mass detection. This chapter focuses on MALDI MSI of peptides and provides detailed operational instructions for sample preparation of cryoconserved and formalin-fixed paraffin-embedded (FFPE) tissue. Besides sample preparation we provide protocols for the MALDI measurement, tissue staining, and data analysis. On-tissue digestion and matrix application are described for two different commercially available and commonly used spraying devices: the SunCollect (SunChrom) and the ImagePrep (Bruker Daltonik GmbH). |
| Databáze: | MEDLINE |
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