Simultaneous determination of three species with a single-injection step using batch injection analysis with multiple pulse amperometric detection.

Autor: Freitas JM; Instituto de Química - Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, MG, Brazil., Oliveira Tda C; Instituto de Química - Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, MG, Brazil., Gimenes DT; Instituto de Química - Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, MG, Brazil., Munoz RA; Instituto de Química - Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, MG, Brazil., Richter EM; Instituto de Química - Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, MG, Brazil. Electronic address: emrichter@iqufu.ufu.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2016 Jan 01; Vol. 146, pp. 670-5. Date of Electronic Publication: 2015 Jun 20.
DOI: 10.1016/j.talanta.2015.06.048
Abstrakt: In this work, the possibility of simultaneous determination of three compounds with a single-injection step using batch injection analysis with multiple pulse amperometric detection (BIA-MPA) is demonstrated for the first time. A sequence of three potential pulses (+1.25 V, +1.60 V, and +1.80 V) was applied with the acquisition of three separate amperograms. 8-Chlorotheophylline was detected selectively at +1.25 V, both 8-chlorotheophylline and pyridoxine at +1.60V and 8-chlorotheophylline, pyridoxine, and diphenhydramine at +1.80 V. Subtraction between the currents detected at the three amperograms (with the help of correction factors) was used for the selective determination of pyridoxine and diphenhydramine. The proposed method is simple, inexpensive, fast (60 injections h(-1)), and present selectivity for the determination of the three compounds in pharmaceutical samples, with results similar to those obtained by HPLC (95% confidence level).
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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