Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba.

Autor: Jerez Puebla LE; Department of Parasitology, Tropical Medicine Institute 'Pedro Kourí', Autopista Novia del Mediodía Km 6 e/Autopista Nacional y Carretera Central, Habana, Cuba., Núñez FA; Department of Parasitology, Tropical Medicine Institute 'Pedro Kourí', Autopista Novia del Mediodía Km 6 e/Autopista Nacional y Carretera Central, Habana, Cuba., Martínez Silva I; Paediatric Hospital 'William Soler', Avenida 100 y Perla, Altahabana, Boyeros, Habana, Cuba., Rojas Rivero L; Department of Parasitology, Tropical Medicine Institute 'Pedro Kourí', Autopista Novia del Mediodía Km 6 e/Autopista Nacional y Carretera Central, Habana, Cuba., Martínez González M; Paediatric Hospital 'William Soler', Avenida 100 y Perla, Altahabana, Boyeros, Habana, Cuba., Méndez Sutil Y; Department of Parasitology, Tropical Medicine Institute 'Pedro Kourí', Autopista Novia del Mediodía Km 6 e/Autopista Nacional y Carretera Central, Habana, Cuba., Ayllón Valdés L; Paediatric Hospital 'William Soler', Avenida 100 y Perla, Altahabana, Boyeros, Habana, Cuba., Atencio Millán I; Department of Parasitology, Tropical Medicine Institute 'Pedro Kourí', Autopista Novia del Mediodía Km 6 e/Autopista Nacional y Carretera Central, Habana, Cuba., Müller N; Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Jazyk: angličtina
Zdroj: Journal of parasitology research [J Parasitol Res] 2015; Vol. 2015, pp. 378643. Date of Electronic Publication: 2015 Nov 29.
DOI: 10.1155/2015/378643
Abstrakt: Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children from La Habana between January and December 2013. Two assemblage-specific PCRs were carried out for the molecular characterization. The overall prevalence of Giardia infection was 11.9%. DNA from 63 of 76 (82.9%) samples was successfully amplified by PCR-tpi, while 58 from 76 (76.3%) were detected by PCRE1-HF. Similar results by both PCRs were obtained in 54 from 76 samples (71%). According to these analyses, assemblage B and mixed assemblages A + B account for most of the Giardia infections in the cohort of children tested. Our current study identified assemblage B as predominant genotype in children infected with Giardia. Univariate analysis indicated that omission of washing hands before eating and keeping dogs at home were significant risk factors for a Giardia infection. In the future, novel molecular tools for a better discrimination of assemblages at the subassemblages level are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis.
Databáze: MEDLINE
Externí odkaz: