Production of Brazilian human norovirus VLPs and comparison of purification methods.

Autor:	Lamounier TA; Departamento de Farmácia, Universidade de Brasília, Brasília, DF, Brazil., de Oliveira LM; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., de Camargo BR; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Rodrigues KB; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Noronha EF; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Ribeiro BM; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Nagata T; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2015 Oct-Dec; Vol. 46 (4), pp. 1265-8. Date of Electronic Publication: 2015 Oct 09.
DOI:	10.1590/S1517-838246420140925
Abstrakt:	Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Databáze:	MEDLINE
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