Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11.

Autor:	Kim JY; Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.; Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore., Sahu S; Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore., Yau YH; School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore., Wang X; Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore., Shochat SG; School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore., Nielsen PH; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark., Dueholm MS; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark., Otzen DE; Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University , 8000 Aarhus C, Denmark., Lee J; Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore., Delos Santos MM; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore., Yam JK; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.; Interdisciplinary Graduate School, Nanyang Technological University , 637551, Singapore., Kang NY; Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore., Park SJ; Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore., Kwon H; Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore., Seviour T; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore., Yang L; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.; School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore., Givskov M; Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.; Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen , Blegdamsvej 3B, DK-2200 Copenhagen, Denmark., Chang YT; Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.; Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2016 Jan 13; Vol. 138 (1), pp. 402-7. Date of Electronic Publication: 2016 Jan 04.
DOI:	10.1021/jacs.5b11357
Abstrakt:	Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.
Databáze:	MEDLINE
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