| Autor: |
Sarkisyan KS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia., Zlobovskaya OA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia., Gorbachev DA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.; Faculty of Biology, Moscow State University, Moscow, Russia., Bozhanova NG; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia., Sharonov GV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.; Faculty of Medicine, Moscow State University, Moscow, Russia., Staroverov DB; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia., Egorov ES; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.; Pirogov Russian National Research Medical University, Moscow, Russia., Ryabova AV; Laser Biospectroscopy Laboratory, Prokhorov General Physics Institute, Moscow, Russia., Solntsev KM; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332, United States of America., Mishin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia., Lukyanov KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia. |
| Abstrakt: |
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity. |
