A rapid PCR assay to characterize the intact pks15/1 gene, a virulence marker in Mycobacterium tuberculosis.

Autor: Zenteno-Cuevas R; Instituto de Salud Pública, Universidad Veracruzana, Veracruz, Mexico. Electronic address: robzencue@gmail.com., Hernandez-Morales RJ; Instituto de Salud Pública, Universidad Veracruzana, Veracruz, Mexico., Pérez-Navarro LM; Dirección de Investigación, Hospital General de México 'Dr. Eduardo Liceaga', México, D.F., Mexico., Muñiz-Salazar R; Laboratorio de Epidemiología y Ecología y Molecular, Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Baja California, Mexico., Santiago-García J; Instituto de Investigaciones Biológicas, Universidad Veracruzana, Veracruz, México.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2016 Feb; Vol. 121, pp. 33-5. Date of Electronic Publication: 2015 Dec 08.
DOI: 10.1016/j.mimet.2015.12.006
Abstrakt: Intact pks15/1 is involved in the biosynthesis of phenolic glycolipids and proposed as a marker for virulence and phylogeny in tuberculosis. Identification of intact condition is achieved mainly by DNA sequencing. For this reason the aim of this study was to develop a reproducible endpoint PCR-assay to characterize it.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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