Identification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle.

Autor:	Blecha IM; Universidade Estadual de Mato Grosso do Sul, Aquidauana, MS, Brasil., Siqueira F; Embrapa Gado de Corte, Campo Grande, MS, Brasil., Ferreira AB; Embrapa Gado de Corte, Campo Grande, MS, Brasil., Feijó GL; Embrapa Gado de Corte, Campo Grande, MS, Brasil., Torres Junior RA; Embrapa Gado de Corte, Campo Grande, MS, Brasil., Medeiros SR; Embrapa Gado de Corte, Campo Grande, MS, Brasil., Sousa II; Universidade Anhanguera Uniderp, Campo Grande, MS, Brasil., Santiago GG; Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., Ferraz AL; Universidade Estadual de Mato Grosso do Sul, Aquidauana, MS, Brasil.
Jazyk:	angličtina
Zdroj:	Genetics and molecular research : GMR [Genet Mol Res] 2015 Dec 09; Vol. 14 (4), pp. 16353-63. Date of Electronic Publication: 2015 Dec 09.
DOI:	10.4238/2015.December.9.3
Abstrakt:	Single nucleotide polymorphisms (SNPs) were screened in FABP3 and FABP4 by automatic sequencing of pools of DNA from crossbred animals whose phenotypes belonged to the upper and lower extremes for back fat and marbling, as well as of a pool of DNA from sires used for crossbreeding. Five SNPs were identified in FABP3 and another nine SNPs were identified in FAPB4. Of these, only one SNP had no previous registry in the SNAP database (dbSNP). Three polymorphisms were selected for further evaluation of their association with production traits using restriction fragment length polymorphism-PCR (RFLP-PCR) or real-time PCR genotyping. All 3 markers were in Hardy-Weinberg equilibrium at the 5% significance level for all 7 genetic groups analyzed. Significant association was observed between FABP3-G/A with rib eye area (P = 0.035) and the rib eye area/hot carcass weight ratio (P = 0.025) and between FABP4/TasI with marbling (P = 0.052) and meat texture (P = 0.053). No significant association was observed between the FABP4-G/C polymorphism and any of the observed traits. Previous association studies with allelic variants in these genes have shown mixed results, probably because of the small effect of the genes for these traits, which suggests that results should be replicated in other populations.
Databáze:	MEDLINE
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