Total energy expenditure assessed by salivary doubly labelled water analysis and its relevance for short-term energy balance in humans.

Autor: Guidotti S; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands.; Groningen Institute for Evolutionary Life Sciences (GELIFES), Unit Behavioral Neuroscience, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands., Verstappen-Dumoulin BM; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands., Jansen HG; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands., Aerts-Bijma AT; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands., van Vliet AA; PRA International, Stationsweg 163, 9471, GP, Zuidlaren, The Netherlands., Scheurink AJ; Groningen Institute for Evolutionary Life Sciences (GELIFES), Unit Behavioral Neuroscience, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands., Meijer HA; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands., van Dijk G; Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands.; Groningen Institute for Evolutionary Life Sciences (GELIFES), Unit Behavioral Neuroscience, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands.
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2016 Jan 15; Vol. 30 (1), pp. 143-50.
DOI: 10.1002/rcm.7412
Abstrakt: Rationale: The doubly labelled water (DLW) method is a stable isotopic technique for measuring total energy expenditure (TEE). Saliva is the easiest sampling fluid for assessing isotopic enrichments, but blood is considered superior because of its rapid exchange with body water. Therefore, we compared a large range of isotopic enrichments in saliva and blood, and related TEE in subjects with their ad libitum total energy intake (TEI). The relevance of these parameters to body weight and fat change over an 8-day interval was also assessed.
Methods: Thirty subjects underwent DLW analysis over either 8 or 14 days, during which time initial and final blood and saliva enrichments were compared. TEI was assessed by dieticians over the 8-day period only. Isotope ratio mass spectrometry was used for the measurement of δ(2)H and δ(18)O values.
Results: No discrepancies were observed between sampling fluids over a wide range of enrichments. During the 8-day period, average TEI exceeded TEE by ~5% or less. Using saliva as sampling fluid, TEI and TEI-TEE, but not TEE, were positively correlated to body weight change. TEI-TEE and physical activity EE (AEE), but not TEI, correlated, respectively, positively and negatively to changes in fat mass.
Conclusions: The DLW method in humans can be reliably applied using saliva as sampling fluid. TEI-TEE as well as AEE contributes significantly to changes in fat mass over an 8-day period.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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