Comparison of biotyping methods as alternative identification tools to molecular typing of pathogenic Cryptococcus species in sub-Saharan Africa.

Autor: Nyazika TK; Department of Chemical Pathology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe.; Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe., Robertson VJ; Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe., Nherera B; Department of Chemical Pathology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe., Mapondera PT; Department of Chemical Pathology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe., Meis JF; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.; Department of Medical Microbiology, Radboudumc, Nijmegen, The Netherlands., Hagen F; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: Mycoses [Mycoses] 2016 Mar; Vol. 59 (3), pp. 151-6. Date of Electronic Publication: 2015 Dec 10.
DOI: 10.1111/myc.12444
Abstrakt: Cryptococcal meningitis is the leading fungal infection and AIDS defining opportunistic illness in patients with late stage HIV infection, particularly in South-East Asia and sub-Saharan Africa. Given the high mortality, clinical differences and the extensive ecological niche of Cryptococcus neoformans and Cryptococcus gattii species complexes, there is need for laboratories in sub-Sahara African countries to adopt new and alternative reliable diagnostic algorithms that rapidly identify and distinguish these species. We biotyped 74 and then amplified fragment length polymorphism (AFLP) genotyped 66 Cryptococcus isolates from a cohort of patients with HIV-associated cryptococcal meningitis. C. gattii sensu lato was isolated at a prevalence of 16.7% (n = 11/66) and C. neoformans sensu stricto was responsible for 83.3% (n = 55/66) of the infections. l-Canavanine glycine bromothymol blue, yeast-carbon-base-d-proline-d-tryptophan and creatinine dextrose bromothymol blue thymine were able to distinguish pathogenic C. gattii sensu lato from C. neoformans sensu stricto species when compared with AFLP genotyping. This study demonstrates high C. gattii sensu lato prevalence in Zimbabwe. In addition, biotyping methods can be used as alternative diagnostic tools to molecular typing in resource-limited areas for differentiating pathogenic Cryptococcus species.
(© 2015 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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