| Autor: |
Narayanan R; Department of Molecular Genetics, Hoffmann-La Roche Incorporated, Nutley, New Jersey 07110., Tare NS, Benjamin WR, Gubler U |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental hematology [Exp Hematol] 1989 Aug; Vol. 17 (7), pp. 832-5. |
| Abstrakt: |
The polymerase chain reaction technique (PCR), a primer-mediated enzymatic amplification of specific target sequences, was used to monitor gene transfer into hematopoietic progenitor stem cells. A gene coding for human interleukin 1 alpha (IL-1 alpha) was cotransfected with the rous sarcoma virus (RSV) CAT plasmid into mouse bone marrow cells by electroporation. Individual chloramphenicol (CAM)-resistant bone marrow progenitor colonies (granulocyte-macrophage colony-forming units; CFU-GM) containing 50-100 cells were analyzed by PCR for the presence and expression of IL-1 alpha and CAT sequences. Amplified IL-1 alpha DNA sequences were detected from a 50-cell CFU-GM colony. CAT and IL-1 alpha RNA expression was demonstrated from the CAM-resistant CFU-GM colonies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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