Increased expression of ERCC2 gene in head and neck cancer is associated with aggressive tumors: a systematic review and case-control study.

Autor:	Zafeer M; Cancer Genetics and Epigenetics Research Group, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad - Pakistan., Mahjabeen I; Cancer Genetics and Epigenetics Research Group, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad - Pakistan., Kayani MA; Cancer Genetics and Epigenetics Research Group, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad - Pakistan.
Jazyk:	angličtina
Zdroj:	The International journal of biological markers [Int J Biol Markers] 2016 Feb 28; Vol. 31 (1), pp. e17-25. Date of Electronic Publication: 2016 Feb 28.
DOI:	10.5301/jbm.5000186
Abstrakt:	Introduction: The excision repair cross-complementation group 2 (ERCC2) ATP-dependent helicase is an essential member of the DNA repair pathway. It has been observed to be differentially expressed in different cancers, which shows its involvement in carcinogenesis. Aim: In the present study we have tried to determine the association of expression patterns of this gene with head and neck carcinogenesis. Method: We first carried out a systematic review of the available studies on the role of ERCC2 in head and neck cancer (HNC). In order to test the hypothesis that the expression patterns of XPD/ERCC2 play a critical role in HNC pathogenesis, we then conducted a population based case-control study on 81 head and neck tumor samples and adjacent normal-tissue control samples. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative polymerase chain reaction (qPCR) were used to assess ERCC2 deregulation at the mRNA level. Result: Expression analysis showed that the ERCC2 expression level was significantly upregulated (p<0.05) in HNC tissues compared with adjacent normal tissues. Furthermore, the expression pattern of ERCC2 was correlated with the expression pattern of Ki-67 and a significant correlation (r = 0.230, p<0.03) was observed between ERCC2 and Ki-67. Spearman's correlation also showed a significant correlation between ERCC2 expression and tumor stage (r = 0.271, p<0.02) and grade (r = 0.228, p<0.02) of HNC. Conclusions: Our data suggest that deregulation of ERCC2 in HNC has the potential to predict a more aggressive cancer phenotype and may be considered a possible biomarker for improved diagnosis and prognosis of HNC.
Databáze:	MEDLINE
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