| Autor: |
de Jesus Coelho Castro R; Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Cidade Universitária CEP 36036-330, Juiz de Fora, MG, Brazil., Sobrinho FS, Sundfeld da Gama MA, Takabayashi Sato R, Lavorato Lima L, Leal de Oliveira MA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2015 Nov-Dec; Vol. 98 (6), pp. 1591-7. |
| DOI: |
10.5740/jaoacint.15-076 |
| Abstrakt: |
A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35®, 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P>0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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