| Autor: |
Sun Joo E; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea., Jin Lee J; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea., Kang MS; Microorganism Resources Division, National Institute of Biological Resources, Incheon 404-107, Republic of Korea., Lim S; Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea., Jeong SW; Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea., Bit Kim E; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea., Hwa Jeon S; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea., Srinivasan S; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea., Kyum Kim M; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea. |
| Abstrakt: |
Three Gram-stain-positive, catalase- and oxidase-positive coccus- or rod-shaped bacterial strains, designated BM2 T , BM4 and BM5, were isolated from soil in South Korea. They showed strong resistance to gamma radiation with a D 10 value of 9 kGy but weak UVC resistance. The 16S rRNA sequences of strains BM2 T , BM4 and BM5 represent a novel subline within the genus Deinococcus in the family Deinococcaceae . The 16S rRNA gene sequences of strains BM2 T , BM4 and BM5 were indistinguishable and showed 98.1-87.3 % similarity with other species of the genus Deinococcus . Strain BM2 T exhibited relatively high levels of DNA-DNA hybridization with BM4 (87 ± 0.8 %) and BM5 (92 ± 1.2 %). Meanwhile, it showed a low level of DNA-DNA hybridization ( < 30 %) with other closely related species of the genus Deinococcus . The strains showed the typical chemotaxonomic characteristics of the genus Deinococcus , with the presence of menaquinone 8 as the respiratory quinone; the major fatty acids were summed feature 3 (composed of C 16 : 1 ω7 c /C 16 : 1 ω6 c ), C 15 : 1 ω6 c and C 16 : 0 . The DNA G+C content of strain BM2 T was 69.7 mol%. The polar lipid profile included major amounts of phosphatidylglycerol, phosphatidylcholine and an unknown aminolipid. On the basis of phenotypic and genotypic properties, and phylogenetic distinctiveness, strains BM2 T , BM4 and BM5 should be classified in a novel species in the genus Deinococcus , for which the name Deinococcus actinosclerus sp. nov. is proposed. The type strain is BM2 T ( = KEMB 5401-184 T = JCM 30700 T ); reference strains are BM4 ( = JCM 30701) and BM5 ( = JCM 30702). |
