Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design.

Autor: de Araújo NK; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Pimentel VC; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., da Silva NM; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., de Araújo Padilha CE; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., de Macedo GR; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Dos Santos ES; Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2016 Feb; Vol. 39 (4), pp. 709-16. Date of Electronic Publication: 2016 Jan 14.
DOI: 10.1002/jssc.201500900
Abstrakt: This study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. A chitosanase-producing strain was isolated and identified as Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorption conditions for chitosanase purification were optimized statistically using STREAMLINE(TM) DEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by the quality criteria purification factor (P) and enzyme yield to optimize the chromatographic process. Statistical analyses showed that the optimum conditions for the maximum P were 150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height. Distributor height had a strong influence on the process, considerably affecting both the P and enzyme yield. Optimizing the purification variables resulted in an approximately 3.66-fold increase in the P compared with the value under nonoptimized conditions. This system is promising for the recovery of chitosanase from B. cereus C-01 and is economically viable because it promotes the reduction steps.
(© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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