Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.

Autor: Sierra RG; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Gati C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Hamburg, Germany., Laksmono H; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Dao EH; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Gul S; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Fuller F; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Kern J; Lawrence Berkeley National Laboratory, Berkeley, California, USA.; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Chatterjee R; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Ibrahim M; Institute für Biologie, Humboldt University of Berlin, Berlin, Germany., Brewster AS; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Young ID; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Michels-Clark T; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Aquila A; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Liang M; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Hunter MS; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Koglin JE; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Boutet S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Junco EA; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Hayes B; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Bogan MJ; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Hampton CY; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Puglisi EV; Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA., Sauter NK; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Stan CA; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Zouni A; Institute für Biologie, Humboldt University of Berlin, Berlin, Germany., Yano J; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Yachandra VK; Lawrence Berkeley National Laboratory, Berkeley, California, USA., Soltis SM; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Puglisi JD; Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA., DeMirci H; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2016 Jan; Vol. 13 (1), pp. 59-62. Date of Electronic Publication: 2015 Nov 30.
DOI: 10.1038/nmeth.3667
Abstrakt: We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
Databáze: MEDLINE
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