Orthotopic transplantation of a tissue engineered diaphragm in rats.

Autor: Gubareva EA; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Sjöqvist S; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Gilevich IV; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Sotnichenko AS; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Kuevda EV; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Lim ML; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Feliu N; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Lemon G; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Danilenko KA; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Nakokhov RZ; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Gumenyuk IS; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation., Grigoriev TE; National Research Centre 'Kurchatov Institute' (NRC 'Kurchatov Institute') Laboratory of Polymer Materials, Moscow, Russian Federation., Krasheninnikov SV; National Research Centre 'Kurchatov Institute' (NRC 'Kurchatov Institute') Laboratory of Polymer Materials, Moscow, Russian Federation., Pokhotko AG; Department of Human Physiology, Kuban State Medical University, Krasnodar, Russian Federation., Basov AA; Common Use Center for Diagnostics of Nanomaterials, Structure and Properties, Kuban State University, Krasnodar, Russian Federation., Dzhimak SS; Common Use Center for Diagnostics of Nanomaterials, Structure and Properties, Kuban State University, Krasnodar, Russian Federation., Gustafsson Y; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Bautista G; College of Medicine, University of Illinois, Chicago, IL, United States., Beltrán Rodríguez A; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Pokrovsky VM; Department of Human Physiology, Kuban State Medical University, Krasnodar, Russian Federation., Jungebluth P; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden., Chvalun SN; National Research Centre 'Kurchatov Institute' (NRC 'Kurchatov Institute') Laboratory of Polymer Materials, Moscow, Russian Federation., Holterman MJ; Department of Surgery, College of Medicine, University of Illinois, Chicago, IL, United States., Taylor DA; Texas Heart Institute, Center for Regenerative Medicine, Houston, TX, United States., Macchiarini P; International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden. Electronic address: paolo.macchiarini@ki.se.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2016 Jan; Vol. 77, pp. 320-35. Date of Electronic Publication: 2015 Nov 14.
DOI: 10.1016/j.biomaterials.2015.11.020
Abstrakt: The currently available surgical options to repair the diaphragm are associated with significant risks of defect recurrence, lack of growth potential and restored functionality. A tissue engineered diaphragm has the potential to improve surgical outcomes for patients with congenital or acquired disorders. Here we show that decellularized diaphragmatic tissue reseeded with bone marrow mesenchymal stromal cells (BM-MSCs) facilitates in situ regeneration of functional tissue. A novel bioreactor, using simultaneous perfusion and agitation, was used to rapidly decellularize rat diaphragms. The scaffolds retained architecture and mechanical properties and supported cell adhesion, proliferation and differentiation. Biocompatibility was further confirmed in vitro and in vivo. We replaced 80% of the left hemidiaphragm with reseeded diaphragmatic scaffolds. After three weeks, transplanted animals gained 32% weight, showed myography, spirometry parameters, and histological evaluations similar to native rats. In conclusion, our study suggested that reseeded decellularized diaphragmatic tissue appears to be a promising option for patients in need of diaphragmatic reconstruction.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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