| Autor: |
Zaripov MM, Afanasieva GV, Glukhova XA, Trizna YA, Glukhov AS, Beletsky IP, Prusakova OV |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2015 Jul-Aug; Vol. 41 (4), pp. 411-5. |
| DOI: |
10.1134/s1068162015030127 |
| Abstrakt: |
A simple and fast method for obtaining biotin-labeled monoclonal antibodies was developed usingcontent of hybridoma culture supernatant sufficient to select antibody pairs in sandwich ELISA. The method consists in chemical biotinylation of antigen-bound antibodies in a well of ELISA plate. Using as an example target Vaccinia virus A27L protein it was shown that the yield of biotinylated reactant is enough to set comprehensive sandwich ELISA for a moderate size panel of up to 25 monoclonal antibodies with an aim to determine candidate pairs. The technique is a cheap and effective solution since it avoids obtaining preparative amounts of antibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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