Cranberry ( Vaccinium macrocarpon ) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli .

Autor: Sun J; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA., Marais JP; Ocean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, MA, 02349, USA., Khoo C; Ocean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, MA, 02349, USA., LaPlante K; Departments of Biomedical and Pharmaceutical Sciences and Pharmacy Practice, College of Pharmacy, University of Rhode Island, Kingston, RI, 02881, USA ; Veterans Affairs Medical Center, Providence, RI, 02908, USA., Vejborg RM; Department of Immunology and Microbiology, University of Copenhagen, DK 2200 Copenhagen N, Denmark., Givskov M; Department of Immunology and Microbiology, University of Copenhagen, DK 2200 Copenhagen N, Denmark., Tolker-Nielsen T; Department of Immunology and Microbiology, University of Copenhagen, DK 2200 Copenhagen N, Denmark., Seeram NP; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA., Rowley DC; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.
Jazyk: angličtina
Zdroj: Journal of functional foods [J Funct Foods] 2015 Aug; Vol. 17, pp. 235-242.
DOI: 10.1016/j.jff.2015.05.016
Abstrakt: The preventive effects of the American cranberry ( Vaccinium macrocarpon ) against urinary tract infections are supported by extensive studies which have primarily focused on its phenolic constituents. Herein, a phenolic-free carbohydrate fraction (designated cranf1b-F2) was purified from cranberry fruit using ion exchange and size exclusion chromatography. MALDI-TOF-MS analysis revealed that the cranf1b-F2 constituents are predominantly oligosaccharides possessing various degrees of polymerisation and further structural analysis (by GC-MS and NMR) revealed mainly xyloglucan and arabinan residues. In antimicrobial assays, cranf1b-F2 (at 1.25 mg/mL concentration) reduced biofilm production by the uropathogenic Escherichia coli CFT073 strain by over 50% but did not inhibit bacterial growth. Cranf1b-F2 (ranging from 0.625 - 10 mg/mL) also inhibited biofilm formation of the non-pathogenic E. coli MG1655 strain up to 60% in a concentration-dependent manner. These results suggest that cranberry oligosaccharides, in addition to its phenolic constituents, may play a role in its preventive effects against urinary tract infections.
Databáze: MEDLINE
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